fni [2017-8-11 16:0:3]

isopentenyl diphosphate isomerase, last (8th) step in the MEP pathway of isoprenoid biosynthesis

locus: BSU22870

pI: 6.10

mw: 22.45 kDa

protein length: 212 aa

gene length: 636 bp

function: MEP pathway of isoprenoid biosynthesis

product: isopentenyl diphosphate isomerase

essential: no

ec: 5.3.3.2

synonyms: ypgA, idi

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]

Gene

Coordinates: 2,393,602 → 2,394,651

The protein

Catalyzed reaction/ biological activity

• Isopentenyl diphosphate = dimethylallyl diphosphate (according to Swiss-Prot)

Protein family

• IPP isomerase type 2 family (according to Swiss-Prot)

Structure

• [PDB|1P0N] (complex with FMN)
• [PDB|1P0K]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
  description: [pubmed|22383849]

• genes: [gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
  description: [pubmed|22383849]

• genes: [gene|C194336ED96FA844FFE9FB43BE59ADC5D6DDB3BE|motI]-[gene|3459BA0CDAABC288ADF97B111F78C7EAC184840D|ypfB]-[gene|1ADB6E2C8DC82A242CFEFAD22443D14226F1BA60|cmk]-[gene|7C36409F155E4C91CEE902FE6937571BD0C50128|ypfD]-[gene|E91624382EC3DAABFB3123CDA818B4BEF745ACE6|fni]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A420 (ypgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/420 NBRP B. subtilis, Japan]

References

• 12798687,17458547,14745175,23840410